Table 2 Assay conditions for each of the nine genes evaluated.
Gene symbol | Primers 5'-3' (forward, reverse) | Amplicon length, bp | PCR efficiency, % | Correlation with dilution series (R2) |
|---|---|---|---|---|
ACTB | GGACCTGACGGACTACCTC CACGCACGATTTCCCTCTC | 83 | 95.2 | 0.999 |
B2M | CCTGCTCGGGCTACTCTC CATTCTCTGCTGGGTGACG | 89 | 100.2 | 1.000 |
GAPDH | ATCTGACCTGCCGCCTGGAG CGATGCCTGCTTCACCACCTTC | 68 | 102.6 | 1.000 |
HPRT | AATTATGGACAGGACTGAACGG ATAATCCAGCAGGTCAGCAAAG | 121 | 93.9 | 1.000 |
R18S | GTCTGCCCTATCAACTTTCG TTCCTTGGATGTGGTAGCC | 119 | 94.1 | 0.998 |
RPL32 | GGGAGCAATAAGAAAACGAAGC CTTGGAGGAGACATTGTGAGC | 138 | 97.0 | 1.000 |
SDHA | GAGGAATGGTCTGGAATACTG GCCTCTGCTCCATAAATCG | 91 | 96.0 | 0.999 |
TFRC | TGGCTACTTGGGCTATTGTAAACG GGTGGTTCTGTTCCCTCTATCTCC | 90 | 97.6 | 0.998 |
UBB | TTCGTGAAGACCCTGACC CCTTATCCTGGATCTTGGC | 91 | 99.4 | 0.999 |