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Table 1 List of Primers and PCR conditions used for 5' RACE

From: Distinct and stage specific nuclear factors regulate the expression of falcipains, Plasmodium falciparum cysteine proteases

Gene

Primers

Primer sequences 5'-3'

PCR conditions

   

Denaturation

Annealing

Extension

Falcipain 1

Fal1RACE GSP1R

CAAGATAGGATAGAAGACTTCCTC

   
 

Fal1RACE GSP2R

CATCTTCTTCATTGGAGGATTC

94°C 1'

52°C 1.30'

68°C 1.30'

 

Fal1RACE GSP3R

CCATGTTGTAATCCATGGTCTTCAAC

94°C 1'

50°C 1.30'

68°C 1.30'

Falcipain 2

Fal2RACE GSP1R

TTACCTTAATGGAATGAATGCATC

   
 

Fal2RACE GSP2R

TAGGAAAATAAAATAAAACAAAACCAA

94°C 1'

52°C 1'

68°C 1.30'

 

Fal2RACE GSP3R

CATGTGGTAATCCATGGTTAAAA

94°C 1'

52°C 1.30'

68°C 1.30'

Falcipain 2a

Fal2aRACE GSP1R

TTACCTTAATGGAATGAATGCATC

   
 

Fal2aRACE GSP2R

TAGGAAAATAAAATAAAACAAAACCAA

94°C 1'

52°C 1'

68°C 1.30'

 

Fal2aRACE GSP3R

TATGAATATACATATACACTAGG

94°C 1'

50°C 1.30'

68°C 1.30'

Falcipain 3

Fal3RACE GSP1R

TTCAAGTAATGGTACATAAGCTTC

   
 

Fal3RACE GSP2R

CCTAGGATCGTTTATATTATTTGATAA

94°C 1'

52°C 1'

68°C 1.30'

 

Fal3RACE GSP3R

CATATGATATTCCATGGTTCAAAC

94°C 1'

50°C 1.30'

68°C 1.30'

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BMC Molecular Biology

ISSN: 1471-2199

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