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Table 2 Efficiency data for candidate reference genes

From: Identification of valid reference genes for the normalization of RT qPCR gene expression data in human brain tissue

Gene Symbol Slope R2 Efficiency
UBC -3.332 0.996 99%
YWHAZ -3.345 0.998 99%
RP II -3.412 0.9995 96%
HMBS -3.294 0.997 100%
TBP -3.317 0.9944 100%
B2M -3.452 0.9992 95%
GAPDH -3.519 0.999 92%
SDHA -3.456 0.9985 95%
  1. Ct values obtained from 10-fold serial dilutions of pooled cDNA (from ×10 dilution to ×100000 dilution) were plotted against dilution factors. The reaction efficiency was calculated using the equation E = 10(-1/slope) where 'E' is the efficiency and 'slope' is the gradient of the best fit line.