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Table 2 Efficiency data for candidate reference genes

From: Identification of valid reference genes for the normalization of RT qPCR gene expression data in human brain tissue

Gene Symbol

Slope

R2

Efficiency

UBC

-3.332

0.996

99%

YWHAZ

-3.345

0.998

99%

RP II

-3.412

0.9995

96%

HMBS

-3.294

0.997

100%

TBP

-3.317

0.9944

100%

B2M

-3.452

0.9992

95%

GAPDH

-3.519

0.999

92%

SDHA

-3.456

0.9985

95%

  1. Ct values obtained from 10-fold serial dilutions of pooled cDNA (from ×10 dilution to ×100000 dilution) were plotted against dilution factors. The reaction efficiency was calculated using the equation E = 10(-1/slope) where 'E' is the efficiency and 'slope' is the gradient of the best fit line.
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BMC Molecular Biology

ISSN: 1471-2199

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