Effects of deletion of NBS1 and NBS3 on PCAN1 promoter activity. pGL3-p PCAN1 is a luciferase reporter plasmid containing a 2.6 kb wild type PCAN1 promoter; pGL3-NBS-Id represented pGL3-NBS1id-p PCAN1 with deletion of NBS1 from the PCAN1 promoter; pGL3-NBS-Idd represented pGL3-NBS1,3idd-p PCAN1, in which both NBS1 and NBS3 were deleted from the PCAN1 promoter. They were cotransfected with pcDNA3.1-NKX3.1 (grey bar) or pcDNA3.1 (+) vector (blank bar) into LNCaP cells, and the promoter activity was analyzed by dual-luciferase reporter assays. Results were expressed as relative luciferase activities (M1/M2). The data were represented as the mean of four individual values ± SD. *p < 0.01, **p < 0.05, grey bar vs blank bar.