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Figure 4 | BMC Molecular Biology

Figure 4

From: Characterization of two functional NKX3.1 binding sites upstream of the PCAN1 gene that are involved in the positive regulation of PCAN1 gene transcription

Figure 4

NKX3.1 binds to the NBSs of the PCAN1 promoter in living cells. LNCaP cells transfected with pcDNA3.1-NKX3.1 were cross-linked by formaldehyde treatment and lysed. Cell lysates were subjected to immunoprecipitation with either an antibody to NKX3.1 (A) or rabbit IgG (B). Four primers (names and sequences are shown in Table 1) spanning five NBSs of PCAN1 promoter region were used for PCR of recovered DNA from the immunoprecipitation (lanes 2–5). Input DNA was used as positive control (C).

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BMC Molecular Biology

ISSN: 1471-2199

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