Figure 2From: Characterization of two functional NKX3.1 binding sites upstream of the PCAN1 gene that are involved in the positive regulation of PCAN1 gene transcriptionEffects of NKX3.1 on PCAN1 mRNA expression in LNCaP and PC-3 cells. The effects of NKX3.1 on PCAN1 mRNA expression as detected by RT-PCR. A. PCAN1 mRNA expression following transfection of LNCaP cells with 1. pcDNA3.1-NKX3.1; 2. pcDNA3.1 (+); or following transfection of PC-3 cells with 3. pcDNA3.1-NKX3.1; or 4. pcDNA3.1 (+). B. Relative expression levels were presented as the ratio of densities of NKX3.1 or PCAN1 to β-actin bands. The results were expressed as mean ± SD (n = 3). C. The expression of NKX3.1 protein in LNCaP and PC-3 cells after transfection of pcDNA3.1-NKX3.1 (1, 3, 5, 7) or pcDNA3.1 (+) (2, 4, 6, 8).D. NKX3.1 and PCAN1 mRNA expression following stable transfection of LNCaP cells with 1. pRNAT-RNAi1 targeting human NKX3.1; 2. pRNAT-RiN as a control vector.Back to article page