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Figure 2 | BMC Molecular Biology

Figure 2

From: A GATA4/WT1 cooperation regulates transcription of genes required for mammalian sex determination and differentiation

Figure 2

SRY promoter GATA motifs bind GATA4. An EMSA was performed with recombinant GATA4 protein and a 32P-labeled oligonucleotide probe corresponding to the first consensus GATA motif (GATA site 1) of the pig SRY promoter. Competition with an oligonucleotide containing a mutated GATA motif (GATA to GGTA) was used to confirm the specificity of GATA4 binding (GATAmut site 1). GATA4 binding to the more distal GATA motifs (GATA sites 2–4) was then assessed by competition experiments using the indicated oligonucleotides.

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