Figure 2

SRY promoter GATA motifs bind GATA4. An EMSA was performed with recombinant GATA4 protein and a ³²P-labeled oligonucleotide probe corresponding to the first consensus GATA motif (GATA site 1) of the pig SRY promoter. Competition with an oligonucleotide containing a mutated GATA motif (GATA to GGTA) was used to confirm the specificity of GATA4 binding (GATA_mut site 1). GATA4 binding to the more distal GATA motifs (GATA sites 2–4) was then assessed by competition experiments using the indicated oligonucleotides.