Figure 1From: A GATA4/WT1 cooperation regulates transcription of genes required for mammalian sex determination and differentiationTranscriptional properties of GATA4 on the mouse, pig, and human SRY promoters. A. Multiple consensus (but not species conserved) GATA binding motifs are present in the first 2 kilobases of the mouse, human, and pig SRY promoters; the GATA sites are indicated by the lozenges. B. Ability of GATA4 to transactivate the SRY promoter. HeLa cells were co-transfected with either a -1090 bp mouse, -1400 bp pig, or -1250 bp human SRY-luciferase promoter construct (500 ng) along with increasing amounts of a GATA4 expression vector (25, 50, or 100 ng). All promoter activities are reported as fold activation over control ± S.E.M. Like letters indicate no statistically significant difference between groups (P > 0.05). C. Western blot analysis of nuclear extracts (10 μg) from HeLa cells overexpressing GATA4.Back to article page