Figure 4From: Molecular characterization of senescence marker protein-30 gene promoter: Identification of repressor elements and functional nuclear factor binding sitesElectrophoretic mobility shift assay to confirm the binding of C/EBP transcription factor to DNase I protected site FP 4: Lane 1 , labeled FP 4 oligonucleotide duplex with 6 μg RLNE; Lane 2 C/EBPβ antibody; Lane 3–5 , describe the competition with 100 fold molar excess of unlabeled homologous self, C/EBP consensus, and nonspecific Oct 1 oligonucleotide duplex respectively. Antibody shift is seen with C/EBPβ antibody.Back to article page