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Figure 17 | BMC Molecular Biology

Figure 17

From: Molecular characterization of senescence marker protein-30 gene promoter: Identification of repressor elements and functional nuclear factor binding sites

Figure 17

Electrophoretic mobility shift assay to confirm the binding of Sp1 transcription factor. Lane 1, labeled mutated SMP-Sp1 oligonucleotide duplex with 6 μg RLNE; Lane 2–6, labeled SMP-Sp1 oligonucleotide duplex with 6 μg RLNE; Lane 3–6, describe the competition with 100 fold molar excess of unlabeled homologous self, Sp1 consensus, Mut SMP-Sp1 and nonspecific SRY oligonucleotide duplex respectively.

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