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Figure 13 | BMC Molecular Biology

Figure 13

From: Molecular characterization of senescence marker protein-30 gene promoter: Identification of repressor elements and functional nuclear factor binding sites

Figure 13

DNase I protected sites, Luc 3–1, Luc 3–2 and Luc 3–3 were mutated at their core binding sites. Reporter activity of the three mutated construct along with wild type Luc 3 construct is represented on the right site and the schematic representation of the mutated sites along with wild type Luc 3 on left site. All transfections were repeated in duplicates and the results are expressed as the mean of three different experiments ± S.D Approximate location of the transcription factor binding to wild type Luc 3 and site directed mutated construct are shown. Mutation of site 3 shows a significant (~59%) increase in luciferase activity as compared to wild type Luc3.

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