Mutation detection using ENDO1: Application to disease diagnostics in humans and TILLING and Eco-TILLING in plants

Table 1 Primers used to amplify target loci

Targets	Primer name	Primer sequence
Factor V	M13F-FV Forward	cacgacgttgtaaaacgac ttaattggttccagcgaaagc
	M13R-FV Reverse	ggataacaatttcacacagg tccaataccaacagacctgg
Reverse Trans.	M13F-RT Forward	cacgacgttgtaaaacgac taggacctacacctgtcaacataa
	M13R-RT Reverse	ggataacaatttcacacagg cttctgtatgtcattgacagtcca
RBR exon10-11	RB-1N1 Forward	ggagcagtcagaataagcggaatg
	RB-1N1 Reverse	taaccaagcccacatcgacgtcatc
	RB-1N2 Forward	cacgacgttgtaaaacgac ggctacaattatctcagcagataag
	RB-1N2 Reverse	ggataacaatttcacacagg gtcctcttactttccggttgctgaa
RBR exon14-17	RB-2N1 Forward	tgaagaagacgccctgtttggcatc
	RB-2N1 Reverse	gttgcttaagtgaagaagccgatct
	RB-2N2 Forward	cacgacgttgtaaaacgac tgagtgtcttggaaaatgattacga
	RB-2N2 Reverse	ggataacaatttcacacagg aggcaaggaaggcacaggaggcaat
BAC F15K9	Tail-At Forward	cacgacgttgtaaaacgac gctgattatgggatgatgatatt
	Tail-At Reverse	ggataacaatttcacacagg aaaaagcagcgagttataggtttac

Targets: corresponding GenBank accession numbers are Human coagulation factor V (Factor V, Z99573), HIV-1 Reverse Transcriptase (Reverse Trans, AF033819), Pisum sativum Retinoblastoma-related gene RBR (AB012024) and Arabidopsis thaliana BAC F15K9 sequence (AC005278).
Primer sequence: in bold are indicated M13 forward and reverse universal tails; in simple text is indicated the part of the primer specific to the target sequence.

ISSN: 1471-2199