Figure 4
From: Gene transfer of Hodgkin cell lines via multivalent anti-CD30 scFv displaying bacteriophage
Cell binding studies of M13-pAK8-Ki-4(EGFP), M13-pC6-Ki-4(EGFP) and M13-pAK8(EGFP) phagemid particles. 2,5 × 105 of indicated cells (filled black) were incubated with 2 × 1012 phagemid particles (M13-pAK8-Ki-4(EGFP) black line, M13-pC6-Ki-4(EGFP) gray line or M13-pAK8(EGFP) dark gray line) and subsequently incubated with mouse anti-M13 IgG and Alexa Fluor 488-conjugated anti-mouse IgG antibody. Mean fluorescence intensity of cells was analyzed by fluorescence-activated cell sorting (FACS).