Figure 1

Phagemid vectors pCANTAB6-Ki-4(EGFP), pAK8-Ki-4(EGFP) and pAK8(EGFP). Schematic drawing of phagemid vectors and deduced phagemid particles used for gene transfer. pCANTAB6-Ki-4(EGFP) (a) and pAK8-Ki-4(EGFP) (b) contain the pIII signal sequence (S) adjacent to the Ki-4 scFv gene. pCANTAB6-Ki-4(EGFP), pAK8-Ki-4(EGFP) and pAK8(EGFP) (c) contain the enhanced green fluorescence protein gene (EGFP), under the control of a cytomegalovirus promoter (CMV) and terminated by a poly adenylation site (pA), as well as an M13 origin of replication site, with a DNA packaging signal sequence (M13 ori), an ampicillin resistance gene (amp-r) and an E. coli origin of replication site (E. coli ori).