Figure 8

uri mutant cells accumulate DNA damage. A, TUNEL labelling and B, DNA labelling, of a testis from a somatically-rescued uri mutant larva. Strong TUNEL staining was found on the DNA of spermatogonia (large arrow) and early primary spermatocytes (small arrow). Lower levels of TUNEL staining are seen in the oldest cysts present in these testes (mature primary spematocytes, arrowhead). C, D, wild type control larval testes show only background cytoplasmic TUNEL staining. E, F, TUNEL staining is seen on DNA of wild type larval testis cells after treatment with DNAse I (positive control). The large nucleus in this figure (arrow) is from the fat body.