Figure 5

Uri is predominantly cytoplasmic, and has a speckled distribution pattern in testes and embryos. Immunofluorescence showing Uri localisation (A, green in C) to cytoplasmic speckles in wild type primary spermatocytes (A-C). The nuclear envelopes are labelled with anti-lamin antibodies (B, red in C). D-F, Salivary gland cells stained for Uri (D, green in merge) and DNA (E, red in merge) revealing that Uri is predominantly cytoplasmic, and is concentrated in the perinuclear region. G-I, Uri (G, green in I) is also localised to cytoplasmic speckles in interphase cells of cellularised early embryos. Cellular structure and mitotic regions were revealed by anti-tubulin staining (H, red in I). In cycle 14 mitotic domains (upper left region of figure) the Uri staining remained speckled, but was distributed uniformly in the cells.