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Figure 4 | BMC Molecular Biology

Figure 4

From: Drosophila Uri, a PP1α binding protein, is essential for viability, maintenance of DNA integrity and normal transcriptional activity

Figure 4

uri expression is highest in embryos, pupae and adult gonads. A, Western blot showing Uri expression in extracts from various Drosophila developmental stages and tissues. Testis and ovary lanes contain gonads dissected from adults, carcass-testis is the male carcass after testes have been removed by dissection, similarly, carcass-ovary is female after ovaries have been removed. B, RNA in situ hybridisation to testis, the apical region of one testis is shown. uri expression extends from spermatogonia near the apical tip, to early primary spermatocytes, and declines in more mature cells further from the apical tip. C, Western blot showing endogenous Uri in wild type (OreR) wing disc extracts. HisMyc-Uri (HM-Uri) runs at a higher molecular weight, so two bands are present when this construct is expressed in a wild type background. Only the HM-Uri band was detected when HM-Uri was expressed in a uri110b/Df(2R)Px2 background, confirming that the mutant allele does not code for full length Uri protein. Arm protein is shown as a loading control.

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