Table 2 Primers used in RT-PCR assays
Gene | Sense primer | Antisense primer | Amplified product (bp) | Tm (°C) |
|---|---|---|---|---|
Ehmre11 | 5'-CGAGAAGAAGAGCAGCTCAA | 5'-CTTTCCTTTTTCTTCAGCCA | 391 | 49.5 |
Ehrad50 | 5'-CAGCCCAAGACATTCAAACA | 5'-CTGCATAATTGTTGTGCCAA | 277 | 49.5 |
Ehnbs1 | 5'-CACCTCCCACACCACAGTAT | 5'-CTCCACCAATGAATGACCAT | 549 | 49.0 |
Ehrad51 | 5'-ATTGCTTTTACACCAAAG | 5'-TTCTTCTGAATTTAATCC | 400 | 49.5 |
Ehrad51C | 5'-CCACATGACATTGTGAGTCT | 5'-GAATTATCCGATGAAGTGCT | 155 | 45.0 |
Ehrad52 | 5'-ATGACTGAAATAGATACCTC | 5'-AATTTGATTGTTTTAAGAAT | 730 | 37.5 |
Ehrad54 | 5'-GTCATGCCATTGACCAATTA | 5'-TCACACTCTTCCTCAGTTGG | 499 | 47.5 |
Ehrad54b | 5'-GGGCAAAAAATTCACCTAAA | 5'-GTCGTGATCCTCCAAGTGCT | 876 | 50.0 |
Ehrad52/22 | 5'-ATGTCTCATGAAATAAAACCAC | 5'-TCATTTCTTACGTCTAACTATTACT | 516 | 44.5 |
25S rRNA | 5'-TATCAAATCAAAGGACCCGCT | 5'-AAAAGA AAAACTAAGCGGTAA | 631 | 51.0 |
actin | 5'-AGCTGTTCTTTCATTATATGC | 5'-TTCTCTTTCAGCAGTAGTGGT | 220 | 48.0 |