Figure 3From: Transcriptional profile of the homologous recombination machinery and characterization of the EhRAD51 recombinase in response to DNA damage in Entamoeba histolyticamRNA expression profiles of E. histolytica RAD52 epistasis group related genes. A. Ethidium bromide stained 6% PAGE showing the RT-PCR products obtained from 1 μg of total RNA of non-irradiated (No UV-C, lane 1) or irradiated trophozoites harvested at different times (UV-C; lane 2, 0.5 h; lane 3, 3 h and lane 4,12 h). Arrowheads denote the length (bp) of each expected amplified internal fragment, as described in Table 2. B. Densitometric analyses of RT-PCR products in A. Pixels corresponding to the 25S rRNA product were taken as 100% in each lane. Data are the mean of three independent assays.Back to article page