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Figure 2 | BMC Molecular Biology

Figure 2

From: Transcriptional profile of the homologous recombination machinery and characterization of the EhRAD51 recombinase in response to DNA damage in Entamoeba histolytica

Figure 2

Identification and immunodetection of phosphorylated EhH2AX histones (γEhH2AX). A. Multiple sequence alignments of E. histolytica and H. sapiens H2AX histones sequences. Black boxes, identical residues; grey boxes, conserved substitutions; open box, C-terminal SQ motif. Arrowhead denotes the potential phosphorylable serine residue (S156). Numbers at left are relative to the position of the initial methionine in each protein. B. Immunodetection of phosphorylated γEhH2AX polypeptides by Western blot assays using anti-human γH2AX polyclonal antibody (upper panel). CE, cytoplasmic extracts; NE nuclear extracts. Lanes 1 and 2, non-irradiated (No UV.C) trophozoites; lanes 3 and 4, irradiated trophozoites (10 min after UV-C treatment); lanes 5 and 6, irradiated (UV-C) trophozoites (30 min after UV-C treatment). Controls using anti-EhPAP and anti-actin antibodies (middle and bottom panels, respectively) are shown.

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BMC Molecular Biology

ISSN: 1471-2199

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