Table 2 Cross reaction of the miR-Q let-7 assays at different target concentrations. The percentage of cross reaction values was calculated between assay-specific and unspecific miRNA targets, while concentration of assay-specific molecules represented the full value.
let-7a miR-Q | let-7b miR-Q | let-7c miR-Q | |
|---|---|---|---|
2 nM let-7a | 100 | 0.31 | 0.01 |
2 nM let-7b | 0.00 | 100 | 0.23 |
2 nM let-7c | 0.37 | 0.29 | 100 |
200 pM let-7a | 100 | 0.31 | 0.01 |
200 pM let-7b | 0.00 | 100 | 0.13 |
200 pM let-7c | 0.24 | 0.24 | 100 |
20 pM let-7a | 100 | 0.30 | 0.01 |
20 pM let-7b | 0.00 | 100 | 0.10 |
20 pM let-7c | 0.24 | 0.19 | 100 |
2 pM let-7a | 100 | 0.00 | 0.00 |
2 pM let-7b | 0.00 | 100 | 0.00 |
2 pM let-7c | 0.00 | 0.16 | 100 |
200 fM let-7a | 100 | 0.00 | 0.00 |
200 fM let-7b | 0.00 | 100 | 0.00 |
200 fM let-7c | 0.00 | 0.00 | 100 |
20 fM let-7a | 100 | 0.00 | 0.00 |
20 fM let-7b | 0.00 | 100 | 0.00 |
20 fM let-7c | 0.00 | 0.00 | 100 |
2 fM let-7a | 100 | 0.00 | 0.00 |
2 fM let-7b | 0.00 | 100 | 0.00 |
2 fM let-7c | 0.00 | 0.00 | 100 |