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Figure 2 | BMC Molecular Biology

Figure 2

From: The katG mRNA of Mycobacterium tuberculosis and Mycobacterium smegmatis is processed at its 5' end and is stabilized by both a polypurine sequence and translation initiation

Figure 2

In vitro transcription. In vitro transcription was performed with 10 μg of crude M. smegmatis extract in the presence of [α-32P]-CTP. The templates were plasmid pMV261, which carries the M. bovis BCG hsp60 promoter, and plasmid pMYS648, in which the 437–552 M. smegmatis region has been substituted to the hsp60 promoter region. The samples were run on a 5% denaturing polyacrylamide gel. Molecular weight markers (MWM) run in the same gel are indicated on the right. The about 300 bp transcript synthesized by pMV261 is indicated by an arrow.

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BMC Molecular Biology

ISSN: 1471-2199

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