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Figure 1 | BMC Molecular Biology

Figure 1

From: The katG mRNA of Mycobacterium tuberculosis and Mycobacterium smegmatis is processed at its 5' end and is stabilized by both a polypurine sequence and translation initiation

Figure 1

The furA - katG region in M. tuberculosis and M. smegmatis. Panel A. Schematic representation of the furA-katG region. Genes are indicated by open boxes, the polypurine sequences (PPS) by a black box. The coordinates of the M. tuberculosis sequence are arbitrary, with coordinate +1 corresponding to the first codon of furA (coordinate 2,156,590 in the reverse strand of the M. tuberculosis complete genome sequence deposited in the GenBank:AL123456). Coordinates of the M. smegmatis sequence are arbitrary, with coordinate +1 corresponding to one bp immediately upstream of the furA start codon (GenBank:NC_008596, [42]). The position of the 5' ends of katG mRNA (+428 in M. tuberculosis, [25]; 443 and 478 in M. smegmatis, [27]) are indicated by vertical arrows below the map. Panel B. Sequence of part of the furA-katG region of M. tuberculosis and M. smegmatis. The 5' ends of the katG mRNA are highlighted in grey, the stop codon of furA and the start codon of katG are in bold. The polypurine sequence upstream of katG is underlined. Panel C. Complementarity between the 16S rRNA of M. tuberculosis and M. smegmatis and the PPS upstream of katG initiation codon. The PPS is in bold and the translation initiation codon is boxed. The accession number of 16S rRNA of M. tuberculosis is GenBank:NC_000962.2 [16] and of M. smegmatis is GenBank:NC_008596 [42].

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BMC Molecular Biology

ISSN: 1471-2199

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