Figure 3From: HMG-box domain stimulation of RAG1/2 cleavage activity is metal ion dependentmtA HMGB1, but not mtB HMGB1, stimulates RAG-mediated 23-RSS hairpin formation in Mg2+, but both proteins promote cleavage under conditions favouring synapsis in Mg2+ and in reactions containing Mn2+. (A-B) In vitro cleavage reactions were performed as in Fig. 2, except that mtA HMGB1 and mtB HMGB1 replaced HMG-box A and HMG-box B in the cleavage reactions.Back to article page