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Figure 4 | BMC Molecular Biology

Figure 4

From: Novel forms of Paired-like homeodomain transcription factor 2 (PITX2): Generation by alternative translation initiation and mRNA splicing

Figure 4

The major PITX2 isoforms in gonadotrope cells, pituitary and skeletal muscle. A) Immunoblot analysis of PITX2 protein expression in CHO cells transfected with different PITX2 isoforms, LβT2 cells, and murine pituitary run on a 12% Nupage® Novex Bis-Tris gel with MOPS buffer. Abbreviations: pcDNA3.0, control; A, PITX2A; B1, PITX2B1; C, PITX2C; Cα, PITX2Cα; Cβ, PITX2Cβ; A+C, PITX2A and PITX2C; nuc, nuclear extract; WCE, whole cell extract. B) PITX2 immunoblot of murine skeletal muscle (Sk. mus.) WCE (lane 1), and LβT2 and αT3-1 nuclear extracts (lane 2 and 3) run on a Nupage® Novex 7% Tris-acetate gel. C) RT-PCR analysis of Pitx2a, Pitx2b1 and Pitx2b2 (top) and Pitx2c (middle) mRNA in LβT2 cells and murine skeletal muscle (hindlimb). Rpl19 (bottom) was used as a loading control.

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BMC Molecular Biology

ISSN: 1471-2199

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