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Figure 3 | BMC Molecular Biology

Figure 3

From: Novel forms of Paired-like homeodomain transcription factor 2 (PITX2): Generation by alternative translation initiation and mRNA splicing

Figure 3

A Novel PITX2 mRNA splice variant. A) Full-length Pitx2a, Pitx2b1, and Pitx2b2 cDNAs amplified by RT-PCR from LβT2 mRNA. B) Schematic representation of the splicing events giving rise to Pitx2b1 (top) or Pitx2b2 (bottom). The 3' end of exon 2 (donor) and 5' end of exon 3 are indicated in white type with black shading. Upper and lower case letters are used for exon and intron nucleotides, respectively. The components of the lariat for the 3' alternative acceptor site are shown, with a branch point (circled, with invariant A shown as bold and italicized), followed by a poly-pyrimidine tract (underlined) and a terminal AG (boxed) at the extreme 3' end. C) RT-PCR analysis of Pitx2a/PITX2A, Pitx2b1/PITX2B1 and Pitx2b2/PITX2B2 mRNAs in LβT2 cells, and murine and human pituitary. D) Immunoblot analysis of PITX2 isoforms expressed in LβT2 cells (left) and combined nuclear extracts from CHO cells (right) transfected with different PITX2 isoforms run on 13.5% Tis-glycine SDS-PAGE gels. A, PITX2A; B1, PITX2B1; B2, PITX2B2; Cα, PITX2Cα; Cβ, PITX2Cβ.

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BMC Molecular Biology

ISSN: 1471-2199

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