Figure 6

E2F1 enhances reporter gene activity of promoter region B. A) The inducible E2F1-expressing Saos2 cells were transfected with deletion constructs of the human DYRK1A promoter as indicated. Five hours after transfection, the medium was changed and cells were treated with doxycycline or were not treated. Data were normalized to β-galactosidase activity and are expressed as fold stimulation relative to untreated cells. The diagram integrates results of 2–6 independent experiments, and bars reflect the means +/- SD. B) Luciferase constructs of the human DYRK1A and the murine Dyrk1a promoter were analyzed for upregulation by doxycycline-induced overexpression of E2F1. Data were normalized to β-galactosidase activity and are presented as the ratio relative to the activity of the unstimulated murine -660 construct. Bars reflect the means +/- SD of 3 independent experiments.