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Figure 4 | BMC Molecular Biology

Figure 4

From: Characterization of the human DYRK1A promoter and its regulation by the transcription factor E2F1

Figure 4

Deletion analysis of the DYRK1A promoter region. Fragments of the 5'-flanking regions of the human DYRK1A gene were fused to the firefly luciferase cDNA in the vector pGL3basic. The position of the promoter fragments relative to TSS B (+1) is indicated. Segments upstream of TSS A and TSS B are highlighted in red and blue, respectively. Saos2 cells were co-transfected with the indicated reporter gene constructs and a β-galactosidase reporter control plasmid. Cells were lysed 48 h after transfection, and luciferase activities were determined from duplicate wells. Data were normalized to β-galactosidase activities and are presented as the ratio relative to the activity of the -742 construct. The diagram integrates results of 2–6 independent experiments. Bars reflect means +/- SD. The inset shows a magnified representation of the weakest signals.

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