Figure 2

RT-PCR analysis of the alternatively spliced DYRK1A transcripts. Forward primers specific for either exon 1A (1Afor) or exon 1B (1Bfor) were used for PCR together with either one of two reverse primers targeting exon 3 (upper panel). First-strand cDNA was subjected to RT-PCR analysis with the indicated primers, and reaction products were visualized by ethidium bromide staining (lower panel). The lengths of the marker bands are indicated in bp. Expected fragment lengths are 192 bp, 232 bp, 216 bp and 256 bp for lanes 1–4. Arrows point to PCR products that were cloned and sequenced.