Table 5 Preamplification primers for SSRT-PreAmp
From: Preamplification techniques for real-time RT-PCR analyses of endomyocardial biopsies
Gene | 5' sense 3' primer | 5' antisense 3' primer |
|---|---|---|
HPRT-CCM | GATATAATTGACACTGGCAAAAC | ACAAACATGATTCAAATCCCTGA |
CD3z | CCGATTACAGAGGCACAGAG | TCGTACTCCTCTCTTCGTCC |
IFNg | TTTTGGGTTCTCTTGGCTGTTA | CTCTTTTGGATGCTCTGGTCA |
FoxP3 | CCCAGAGCTCCTACCCACT | TGGCACTCAGCTTCTCCTTC |
T-bet | GACTGGAGGTGTCGGGGAA | CCACGTCCACAAACATCCTG |
GATA3 | TCACAAAATGAACGGACAGAAC | TAATATTGTGAAGCTTGTAGTAGA |
Granulysin | AGGAGCTGGGCCGTGACTA | CTCTAGACTGATACCTCCTCA |
Perforin | TGTGGCCGGCTCACACTCA | CGTAGTTGGAGATAAGCCTGA |