Table 2 Sequences of primers and probes of self-designed real-time RT-PCR gene assays
From: Preamplification techniques for real-time RT-PCR analyses of endomyocardial biopsies
Gene name | 5' sense 3' primer sequence | 5' antisense 3' primer sequence |
|---|---|---|
TRBC | TCCGCTGTCAAGTCCAGTTCTA | GACAGGACCCCTTGCTGGTA |
TRBV common antisense primer | CTGCTTCTGATGGCTCAAACA | |
TRBV2 | ACTCTGAAGATCCGGTCCACAA | |
TRBV3 | ATCAATTCCCTGGAGCTTGGT | |
TRBV4 | CCTGAATGCCCCAACAGC | |
TRBV5 | GCTCTGAGCTGAATGTGAACGC | |
TRBV5WBL | GCTCTGAGATGAATGTGAGTGC | |
TRBV6 | CACTGACAAAGGAGAAGTCCC | |
TRBV6WBL6-2 | AACTGCCAAAGGAGAGGTCCC | |
TRBV6WBL6-4 | CACTGGCAAAGGAGAAGTCCC | |
TRBV7 | CTCTCAGGTGTGATCCAATTTCG | |
TRBV7WBL7-2;7-3 | AGCTCAGGTGTGATCCAATTTCA | |
TRBV7WBL7-9 | CTTTCAGGTGTGATCCAATTTCT | |
TRBV9 | CACAACAGTTCCCTGACTTGCA | |
TRBV10 | CATGGGCTGAGGCTGATC | |
TRBV11 | CTGCAGAGAGGCTCAAAGGAGTAG | |
TRBV12 | AGAACCCAGGGACTCAGCTGT | |
TRBV13 | GAACTGAACATGAGCTCCTTGGA | |
TRBV14 | CTGAAAGGACTGGAGGGACGTAT | |
TRBV15 | CAGGAGGCCGAACACTTCTTT | |
TRBV16 | GCCTCCCAAATTCACCCTGTA | |
TRBV18 | CCAGCATCCTGAGGATCCA | |
TRBV19 | ACTGTGACATCGGCCCAAA | |
TRBV20 | AACCATGCAAGCCTGACCTT | |
TRBV23 | CCCTGCAGCCTGGCAAT | |
TRBV24 | GCTAAATTCTCCCTGTCCCTAGAGT | |
TRBV25 | TTCCCCTGACCCTGGAGTCT | |
TRBV27 | GGCTTAAGGCAGATCTACTATTCAATG | |
TRBV28 | GCCAGCACCAACCAGACAT | |
TRBV29 | AGCCGCCCAAACCTAACATT | |
TRBV30 | CGGCAGTTCATCCTGAGTTCT | |
HPRT-CCM | AGTCTGGCTTATATCCAACACTTCG | GACTTTGCTTTCCTTGGTCAGG |
IL10 | CAAGTTGTCCAGCTGCTTCAT | TTGGCGAAAGCAGCTCCTC |
IFNγ | CAGGTCATTCAGATGTAGCGGATAA | AGGAGACAATTTGGCTCTGCATT |
TGFβ | CCCTGCCCCTACATTGGAG | CCGGTTATGCTGGTTGTACA |
FoxP3 | TGGCAAACGGAGTCTGCAA | TCTCATCCAAGAGGTGATCTGCTT |
T-bet | CAACACAGGAGGCGCACTGG | CCCCCTTGTTGTTTGTGAGCT |
GATA3 | CCTCATTAAGCCCAAGCGAAG | TTGGCATTTCCTCCAGAGT |
GRAIL | GAAGCAGAGGCAATTAAAGGCAG | CATCAGGGCCAATTTCCTTGT |
Granzyme A | CCCTGTGATTGGAATGAATATGGT | ACACCCTCGCACAACAAAGG |
Granzyme B | GCGAATCTGACTTACGCCATTATT | CAAGAGGGCCTCCAGAGTCC |
Granulysin | AAAACTGAAGAAGATGGTGGATAAGC | ATCGTGACCTCCCCGTCCTA |
Perforin | GGACCAGTACAGCTTCAGCACTG | AGTCAGGGTGCAGCGGG |