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Figure 12 | BMC Molecular Biology

Figure 12

From: Preamplification techniques for real-time RT-PCR analyses of endomyocardial biopsies

Figure 12

T-PreAmp procedure. Principle of the T-PreAmp technique: After standard reverse transcription, cDNA was preamplified (14 cycles) using a pool of all investigated self-designed gene assays (primers) and ABI inventoried Taqman® gene expression assays (mixture of primers and fluorescence probes). The preamplified cDNA products were then diluted 1:20 and subjected to real-time PCR analyses.

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