Figure 5
Binding of ATRX to centromeric heterochromatin and the Y chromosome in neonatal spermatogonia from LSH -/- mice. Neonatal spermatogonia were obtained from heterozygous controls and LSH deficient mice and analyzed by Immuno-FISH. The sub-chromosomal localization of ATRX, H3K9me3, H3K4me2 and 5-mC were correlated with the position of the Y chromosome (red). The patterns of ATRX and H3K9me3 localization to centromeric domains (thin arrows) and the Y chromosome (bold arrow) in cells lacking LSH protein were undistinguishable compared to the patterns observed in heterozygous littermate controls. Neonatal spermatogonial cell nuclei were identified by their unique global DNA methylation patterns (5-mC; green) on the same metaphase spread. Scale bar = 10 μm.