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Table 3 Primer sequences for candidate normalization genes. Amplicon size and efficiency (E) in Senegalese sole and Atlantic halibut are indicated.

From: Selection of housekeeping genes for gene expression studies in larvae from flatfish using real-time PCR

Gene

S. senegalensis

H. hippoglossus

 

Primer Sequence (5'→3')

Amplicon length

E

Primer Sequence (5'→3')

Amplicon length

E

ACTB1

CTCCATCGTCCACCGCAAGTGCTTC (F)

92 bp

0.90

AAGAAGAAAGAAAAGACGGAGGA

81 bp

0.98

 

TGTCCATTCGTGCAAGATCGGGAGA (R)

  

AAGATTTGCCAGAGATGTTTCC

  

ACTB2

AATCGTGACCTCTGCTTCCCCCTGT

113 bp

0.96

GGTGTATGCTGAGCCCAAGA

114 bp

0.98

 

TCTGGCACCCCATGTTACCCCATC

  

CCTGGGACGCTCTGTACTTC

  

GAPDH1

TGTTCGTCTGGAGAAACCCGCCAAA

160 bp

0.94

GTGTCAGTGGTTGACCTGA

212 bp

0.99

 

AGCGCCAGCATCAAAGATGGAGGAG

  

AGCTTGACAAAGTGGTCATT

  

GAPDH2

AGCCACCGTGTCGCCGACCT

107 bp

0.95

   
 

AAAAGAGGAGATGGTGGGGGGTGGT

     

ANXA2

GGTCAACTTCGCAGCGGGAAACCA

69 bp

0.94

CGACTCGCCAAGAAGGATCT

129 bp

0.99

 

CCATGAAGGCTAAAGGGGCAAAGGA

  

CCCTTCATGGAAGCTTTGAG

  

GST

TTTCCAACCATCGCTTATGTCTTCCGTTT

109 bp

0.86

AAGAACCTGCAGGGCTACAA

135 bp

0.99

 

AGCTTTTTTTGATACTGGGCCGGTCCT

  

CCCATGTTTGAAGGAAGGAA

  

ODC

   

GCTTCTGCTTACACGCTAGT

143 bp

1.01

    

TTGAAAGATCCGTACACTCC

  

HPRT1

GGACATGGCGACATCCAGCTCCTGTGTT

120 pb

0.96

GGGGTATGACCTGGACCTTT

108 bp

0.99

 

TGGGGGATGTAGACCCTCTCCAGGTCAG

  

TTGGCCAGTCTCTCTGTCCT

  

UBQ

AGCTGGCCCAGAAATATAACTGCGACA

93 bp

0.95

ATCGAGAATGTCAAGGCTAA

140 bp

0.98

 

ACTTCTTCTTGCGGCAGTTGACAGCAC

  

AGATGCAGAGTGGATTCTTT

  

eEF1A1

GATTGACCGTCGTTCTGGCAAGAAGC

142 bp

0.95

AAGAGGACCATCGAGAAGTT

141 bp

0.97

 

GGCAAAGCGACCAAGGGGAGCAT

  

GTCTCAAACTTCCACAGAGC

  

RPL13a

GCTGCGCTGGAGAGGCTGAAGGTGT

115 bp

0.97

CGGTTCAATAAGGTTCTGCT

111 bp

0.99

 

CTCAAGAAAATACCACAGGATGGGCTTCAA

  

CATCTCACAACCACCACTTT

  

RPS4

GTGAAGAAGCTCCTTGTCGGCACCA

83 bp

0.94

GCCAAGTACAAGCTGTGCAA

138 bp

1.00

 

AGGGGGTCGGGGTAGCGGATG

  

AGGTCGATCTTGACGGTGTC

  

18S rRNA

GAATTGACGGAAGGGCACCACCAG

148 bp

0.97

GGGAGGTAGTGACGAAAAAT

148 bp

1.00

 

ACTAAGAACGGCCATGCACCACCAC

  

AAGATACGCTATTGGAGCTG