Figure 2

F-box and helicase mutants are deficient in DNA damage repair. (A) Wild type and fbh1 Δ, fbh1-fb, fbh1-hl, and fbh1-fb/hl mutant cells were grown in liquid YES. Cells were diluted and spotted onto YES plates with the indicated DNA damaging agents as described in the Methods. The plates were incubated for 3 days at 30°C. (B) Cells were grown and spotted onto YES plates. The plates were incubated for 4 days at 30°C. (C) Cells were grown and spotted onto YES plates containing the indicated concentrations of MMS. The plates were incubated for 3 days at 30°C.