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Table 1 Identification of proteins in the secretome of hMADS cells at early step of adipogenesis and osteogenesis.

From: Characterization of human mesenchymal stem cell secretome at early steps of adipocyte and osteoblast differentiation

ID

Protein name

Function

Mw kDa

Loc

Day 0

Adipo

Osteo

Ref

 

PROTEASES

       

P00736

Complement component 1, r subcomponent

serine protease

80

ex

 

Y

Y

[69]

P09871

Complement component 1, s subcomponent

serine protease

77

sp

 

Y

Y

 

P03956

Matrix metallopeptidase 1 (interstitial collagenase)

collagen catabolism

54

sp

Y

   

P08253

Matrix metallopeptidase 2, gelatinase A (MMP2)

collagen catabolism

74

sp

Y

Y

Y

 

Q15113

Procollagen C-endopeptidase enhancer

collagen catabolism

48

sp

 

Y

  
 

PROTEASE INHIBITORS

       

P05121

Serpin peptidase inhibitor, clade E, plasminogen activator inhibitor type 1(PAI-1)

fibrinolysis

45

sp

Y

 

Y

 

P36955

Serpin peptidase inhibitor, clade F (PEDF)

inhibitor of angiogenesis

46

sp

 

Y

  

P05155

Serpin peptidase inhibitor, clade G (C1 inhibitor), member 1

fibrinolysis

55

sp

Y

Y

  

P01033

TIMP metallopeptidase inhibitor 1

proteolysis inhibitor

23

sp

Y

Y

Y

 
 

EXTRACELLULAR MATRIX COMPONENTS

       

P50454

Serpin H1 precursor (Collagen-binding protein) (Colligin) (47 kDa heat shock protein) (Proliferation-inducing gene 14 protein)

collagen-binding protein

46

sp

Y

   

P98160

Basement membrane-specific heparan sulfate proteoglycan core protein precursor

ECM component

469

sp

Y

 

Y

 

P02452

Collagen, type I, alpha 1

ECM component

139

sp

Y

Y

Y

 

P08123

Collagen, type I, alpha 2

ECM component

129

sp

Y

Y

Y

 

P02461

Collagen, type III, alpha 1

ECM component

138

sp

 

Y

Y

 

P12109

Collagen, type VI, alpha 1

ECM component

108

sp

Y

Y

Y

 

P12110

Collagen, type VI, alpha 2

ECM component

109

sp

Y

Y

Y

 

P12111

Collagen, type VI, alpha 3

ECM component

343

sp

Y

Y

Y

 

P07585

Decorin precursor (Bone proteoglycan II)

ECM component

40

sp

 

Y

  

P02751

Fibronectin precursor

cell growth

26

sp

Y

Y

Y

 

Q08380

Galectin-3-binding protein precursor (Lectin galactoside-binding soluble 3-binding protein)

growth regulation/cell-matrix

65

sp

Y

Y

  

Q16270

Insulin-like growth factor-binding protein 7 precursor (IGFBP-7)

cell adhesion

29

sp

  

Y

 

P11047

Laminin gamma 1 chain precursor (Laminin B2 chain)

ECM component

178

sp

Y

Y

  

P51884

Lumican precursor (Keratin sulfate proteoglycan lumican)

ECM component

38

sp

Y

Y

Y

 

P14543

Nidogen 1

ECM component

136

sp

 

Y

  

Q15063

Periostin, osteoblast specific factor

cell adhesion

93

sp

Y

 

Y

 

Q02809

Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 pecursor

collagen process

83

sp

 

Y

  

O00391

Sulfhydryl oxidase 1 precursor, Quiescin Q6 (QSCN6)

growth regulation

83

sp

  

Y

 

P09486

Secreted protein, acidic, cysteine-rich (osteonectin)

cell-matrix interaction

35

sp

Y

Y

Y

 

Q15582

Transforming growth factor-beta-induced protein ig-h3 precursor (BIGH3)

cell adhesion

75

sp

Y

Y

Y

 
 

ANTI-INFLAMMATORY/ANTI-OXIDANT PROTEINS

       

Q12841

Follistatin-like 1

immunity and defense

35

sp

Y

Y

Y

 

P09211

Glutathione S-transferase P

antioxidant

23

ex

 

Y

 

[70, 71]

P26022

Pentraxin-related gene, rapidly induced by IL-1 beta (PTX3)

inflammatory response

42

sp

Y

Y

Y

 

P30041

PRDX6 peroxiredoxin 6

antioxidant

25

ex

 

Y

 

[72]

Q16881

TXNRD1 thioredoxin reductase 1

antioxidant

55

ex

 

Y

 

[33]

 

METABOLIC ENZYMES

       

Q04828*

Aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1)

progesterone conversion

37

in

 

Y

  

P06733

Enolase 1 (ENO1)

glycolysis

47

ex

Y

Y

Y

[73, 74]

P04406

Glyceraldehyde-3-phosphate dehydrogenase

glycolysis

36

ex

Y

Y

Y

[31]

P40926

Malate dehydrogenase 2, NAD (mitochondrial)

Krebs cycle/gluconeogenesis

36

ex

 

Y

Y

[75, 76]

P00558

Phosphoglycerate kinase 1

glycolysis

44

ex

 

Y

 

[32]

P18669

Phosphoglycerate mutase 1

glycolysis

29

ex

 

Y

 

[70]

P14618

Pyruvate kinase, muscle

glycolysis

58

ex

Y

Y

 

[77]

P29401

Transketolase

pentose phosphate-glycolysis

68

ex

 

Y

Y

[73]

P60174

Triosephosphate isomerase 1

glycolysis

27

ex

 

Y

 

[76]

 

CYTOSKELETAL COMPONENTS

       

P60709

Actin, beta

cell growth

42

ex

Y

Y

Y

[70, 71]

P12814

Actinin, alpha 1

cell growth

103

ex

Y

Y

 

[73, 74]

Q71U36

Alpha-3 tubulin

cell growth

50

ex

Y

Y

 

[73]

Q14019*

Coactosin-like protein

actin-binding protein

16

In

 

Y

  

P15924

Desmoplakin

cell adhesion

332

ex

 

Y

 

[77]

Q16555*

Dihydropyrimidinase-like 2

tubulin binding protein

62

In

 

Y

  

P14923*

Junction plakoglobin

cell adhesion

82

In

 

Y

  

P09382

Lectin, galactoside-binding, soluble, 1 (galectin 1)

growth regulation

15

ex

 

Y

 

[21]

P26038

Moesin

cell-matrix interaction

68

ex

Y

Y

Y

[77]

P07737

Profilin 1

cell growth

15

ex

 

Y

Y

[73]

Q15293*

Reticulocalbin 1, EF-hand calcium binding domain

calcium-binding protein

39

In

 

Y

  

P08670

Vimentin

cell growth

54

ex

  

Y

[71, 73]

P18206

Vinculin

cell growth

124

ex

 

Y

Y

[73]

O75083

WD repeat-containing protein 1 isoform 1 variant

actin pol. control

66

ex

Y

Y

 

[76]

 

HEAT SHOCK/PROTEIN FOLDING PROTEINS

       

P11021

78 kDa glucose-regulated protein precursor (GRP78)

chaperone

72

sp

Y

Y

Y

 

O43852

Calumenin precursor (Crocalbin)

calcium-binding chaperone

37

sp

Y

Y

Y

 

P27797

Calreticulin

calcium-binding chaperone

48

sp

Y

Y

  

P50990*

Chaperonin containing TCP1, subunit 8 (theta)

chaperone

60

In

 

Y

  

P68104

Elongation factor 1-alpha 1 (EF-1-alpha-1)

protein biosynthesis

50

ex

 

Y

 

[78]

P11142

Heat shock 70 kDa protein 8 and homologs

chaperone

71

ex

 

Y

 

[28]

 

Heat shock 70 kDa protein 2 (P54652), Heat shock 70 kDa protein 1-like (P43931)

       

P10809

Heat shock 60 kDa protein 1 (chaperonin)

chaperone

61

ex

 

Y

 

[29]

P07900

Heat shock protein 90 kDa alpha (cytosolic), class A member 1

chaperone

85

ex

 

Y

 

[77]

P30101

PDIA3 protein disulfide-isomerase A3 precursor (EC 5.3.4.1)

protein folding

57

sp

Y

Y

Y

 

P23284

Peptidyl-prolyl cis-trans isomerase B precursor, (Rotamase)

protein folding

23

sp

Y

Y

Y

 

Q15084

Protein disulfide isomerase family A, member 6

protein folding

48

ex

Y

Y

 

[71]

P14625

Endoplasmin precursor, (94 kDa glucose-regulated protein) (GRP94) (gp96 homolog) (Tumor rejection antigen 1)

chaperone

92

sp

 

Y

  
 

OTHER PROTEINS

       

P41250

Glycyl-tRNA synthetase (EC 6.1.1.14) (Glycine – tRNA ligase) (GlyRS)

tRNA synthetase

83

sp

 

Y

  

Q9NTK5*

Putative GTP-binding protein 9 (putative)

GTP-binding protein

45

In

 

Y

  

P50395

RAB GDP dissociation inhibitor beta

vesicular transport

51

sp

 

Y

  

P30153*

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, (Medium tumor antigen-associated 61 kDa protein)

serine/threonine phosphatase

65

In

 

Y

  
  1. Y: indicates the presence of the candidate in the sample; sp: signal peptide; ex: extracellular; in: intracellular; Mw: molecular weight; adipo:day 3 adipocytes; osteo: day 3 osteoblasts; Loc: localisation; ref: reference. * indicates only putatively
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BMC Molecular Biology

ISSN: 1471-2199

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