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Figure 1 | BMC Molecular Biology

Figure 1

From: Characterization of human mesenchymal stem cell secretome at early steps of adipocyte and osteoblast differentiation

Figure 1

Analysis of terminal differentiation of hMADS cells into adipocytes and osteoblasts and Coomassie Blue staining of hMADS cell secretome. A. qRT-PCR analysis of mRNA levels of specific adipogenic (adiponectin) and osteogenic (alkaline phosphatase) markers at day 3 and day 14 of differentiation as compared to day 0. The data are representative of three independent experiments. B. Microphotographs of hMADS cells differentiated into adipocytes and osteoblasts at day 14. Bar scale = 50 μm. C. Representative gel of secreted proteins from hMADS cells at day 0 and day 3 of differentiating adipocytes (adipo) and osteoblasts (osteo) after 6 h of incubation. The gel is representative of 3 independent experiments.

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BMC Molecular Biology

ISSN: 1471-2199

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