Skip to main content
Figure 2 | BMC Molecular Biology

Figure 2

From: The Mre11 protein interacts with both Rad50 and the HerA bipolar helicase and is recruited to DNA following gamma irradiation in the archaeon Sulfolobus acidocaldarius

Figure 2

(a) Analysis of the levels and sub-cellular partitioning of Rad50, Mre11, HerA, NurA and RadA. Immunoblots were performed on total, chromosomal-enriched and soluble fractions recovered after 0, 3, 7 and 24 h of post-treatment growth. C: non-irradiated S. acidocaldarius cells. I: S. acidocaldarius cells exposed to a 1000 Gy irradiation under limited-aerobic conditions. DNA ligase was used as a control of cell fractionation and samples loading. (b) Analysis by gel electrophoresis (1% agarose) of the DNA content of total extract (T), pellet (P), supernatant (S) and pellet from DNase I treated total extract (P +DNase). (c) Western blot analysis of pellets from un-treated (-) and DNase I treated (+) total extracts.

Back to article page

BMC Molecular Biology

ISSN: 1471-2199

Contact us