Figure 6

PCNA ubiquitination decreases after RAD18 knockdown. A) 293T cells were transfected with 100 nM of either control siRNA, MMS2 and UEV1A siRNA or RAD18 siRNA followed by immunoblotting. B) Seventy-two hours post-transfection, cells were subjected to immunoprecipitation with a PCNA antibody and immunoblotted for PCNA (upper panel) and Ub (lower panel). A PCNA immunoblot with darker and lighter exposure performed on protein lysates from the same samples used in the immunoprecipitations is also shown. Asterisks denote immunoglobulin heavy and light chains as detected in the immunoprecipitations.