Figure 1
From: hMMS2 serves a redundant role in human PCNA polyubiquitination
siRNA targeting MMS2 and UEV1a results in an efficient knockdown. (A) 293T cells were transfected with 100 nM of either control siRNA, siRNA MMS2 and siRNA UEV1A followed by immunoblotting. Cells were lysed at the indicated time points and subject to immunoblotting. (B) Hela cells were treated as above and lysed 72 hours post-transfection. (C) 293T cells were treated as in (B).