Skip to main content
Figure 7 | BMC Molecular Biology

Figure 7

From: Ligand-induced sequestering of branchpoint sequence allows conditional control of splicing

Figure 7

Theophylline-mediated modulation of alternative splicing in vivo. (A) HeLa cells were transiently transfected with empty vector, pcABT4M or pcABT4Mmu (containing mutations within core TBA). Cells were treated with buffer (lanes 4 and 6) or with 1 mM theophylline (lanes 5 and 7). The product of RT-PCR assays of total RNA isolated from each well was analyzed on a 2.5% agarose gel to estimate the effect of theophylline on the efficiency of exon 2 alternative splicing. The PCR amplified bands corresponding to exon 2 included or excluded mRNA is indicated. (B) The internal exon 2 exclusion: inclusion ratio for ABT4M or ABT4Mmu in the absence (open box) or presence of theophylline (shaded box) is shown. The data represent mean ± SEM. * P < 0.04 versus mutant is significant.

Back to article page