Figure 5
From: Ligand-induced sequestering of branchpoint sequence allows conditional control of splicing
The location of branchpoint sequence affects efficiency of splicing inhibition. (A) Schematic representation of AdBPT15AG-LS pre-mRNA. The branchpoint sequence is present in the lower aptamer stem and the length of stem is 8 bp. (B) 32P-labeled AdBPT15AG-LS pre-mRNA was incubated in HeLa nuclear extract for 2 h in the absence (lanes 1) or presence of theophylline (lanes 2) as described in Fig. 1. The extracted RNA was fractionated on a 13% polyacrylamide denaturing gel. Percent splicing calculated as in Fig. 1 and values are expressed as mean ± SEM.