A novel mutant of the Sup35 protein of Saccharomyces cerevisiae defective in translation termination and in GTPase activity still supports cell viability

Table 1 In vitro Sup45p- and ribosome-dependent GTPase activity of A and D mutant Sup35pcter.

Components of the incubation mixture	³²P[P_i] released (pmol)	Activity in % compared to the WT
Sup45p	0	-
Sup35pcter wild-type	0	-
Sup35pcter wild-type + Sup45p + ribosomes	2.2	100%
A mutant Sup35pcter + Sup45p + ribosomes	0.14	6%
D mutant Sup35pcter + Sup45p + ribosomes	0.02	1%

ISSN: 1471-2199