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Figure 7 | BMC Molecular Biology

Figure 7

From: A novel mutant of the Sup35 protein of Saccharomyces cerevisiae defective in translation termination and in GTPase activity still supports cell viability

Figure 7

Interaction between Sup45p and wild-type, A or D mutant Sup35 proteins in a two-hybrid system. The entire SUP35 gene was fused to the binding domain of Gal4 (Gal4-BD), and the SUP45 gene to the activating domain of Gal4 (Gal4-AD). Serial dilutions of diploids were spotted on YPD rich medium and on a medium lacking leucine, uracile and histidine with 10 mM 3-aminotriazol to reveal interactions.

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