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Figure 5 | BMC Molecular Biology

Figure 5

From: A novel mutant of the Sup35 protein of Saccharomyces cerevisiae defective in translation termination and in GTPase activity still supports cell viability

Figure 5

The T341D Sup35 protein is not degraded in vivo. For each strain, the same amount of total proteins was separated by SDS-PAGE and western blot analysis shows the amount of Sup35p in each sample (wild-type (WT), A or D mutants). Detection was made using a primary antibody specific of the M domain of Sup35p, and a secondary antibody fused to the horseradish peroxydase.

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BMC Molecular Biology

ISSN: 1471-2199

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