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Figure 4 | BMC Molecular Biology

Figure 4

From: A novel mutant of the Sup35 protein of Saccharomyces cerevisiae defective in translation termination and in GTPase activity still supports cell viability

Figure 4

Full-length T341D compromise viability whereas the C-terminal T341D does not. Both full-length and C-terminal T341A are compatible with viability. The strain deleted for endogenous SUP35 and rescued by an URA3 plasmid containing the wild-type SUP35 gene was transformed with either pRS313-SUP35, pRS313-SUP35A, pRS313-SUP35D (HIS3 plasmids) or with pFL36-SUP35cter, pFL36-SUP35Acter and pFL36-SUP35Dcter (LEU2 plasmids). Transformants were streaked onto 5FOA to eliminate the URA3 plasmid containing the wild-type copy of SUP35.

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BMC Molecular Biology

ISSN: 1471-2199

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