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Table 1 DNA sequences tested for NR2E binding activity

From: Specificity of DNA-binding by the FAX-1 and NHR-67 nuclear receptors of Caenorhabditis elegans is partially mediated via a subclass-specific P-box residue

Site Name

Sequence

% Bound by FAX-1

S.D.

DR1A

AAGTCAaAAGTCA

53.3

13.6

DR1G

AGGTCAaAGGTCA

42.1

11.8

DR1C

ACGTCAaACGTCA

26.1

17.2

DR1T

ATGTCAaATGTCA

29.8

1.9

DRNC

AATTCAaAATTCA

0.4

0.2

MON1

AAGTCAaAATTTA

2.8

0.6

MON2

AATTTAaAAGTCA

4.5

1.0

HRSW

AAGTCAaAATTCA

11.7

1.0

HRWS

AATTCAaAAGTCA

10.5

0.9

DR2A

AAGTCAaaAAGTCA

20.7

6.8

DR3A

AAGTCAaaaAAGTCA

2.3

0.4

  1. The first column indicates the name used to refer to each sequence in the text. The second column shows the actual sequence tested. The third column indicates the percentage of the labeled DNA that was shifted to high mobility in EMSA experiments using FAX-1 protein. The fourth column indicates standard deviation for each set of EMSA experiments summarized. We evaluated the significance of the data using One-Way ANOVA and Fisher 95% Confidence Intervals for all pairwise comparisons. DR1A and DR1G were not significantly different from each other. DR1A was significantly different from all other sequences tested (p < 0.05). The difference between DR1G and DR1C or DR1T fell just outside the range of statistical significance. Binding data for MON1, MON2, DR3A, and DRNC sites were not significantly different from each. Pairwise differences between HRWS or HRSW and MON1 or MON2 were significant (p < 0.05).