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Figure 4 | BMC Molecular Biology

Figure 4

From: Specificity of DNA-binding by the FAX-1 and NHR-67 nuclear receptors of Caenorhabditis elegans is partially mediated via a subclass-specific P-box residue

Figure 4

Binding of FAX-1 to DR2 and DR3 dimeric sites. EMSA of FAX-1 protein binding to DR2A and DR3A sequences (dimeric AAGTCA binding sites separated by two base-pairs and three base-pairs, respectively). Competition experiments with DR2A, DR3A, and DR1A are shown at 10-fold and 100-fold molar excess (wedges) or 10-fold molar excess only (DR3A). We observed additional shifted species in this experiment, however these bands could not be competed with equivalent unlabeled oligonucleotides.

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