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Figure 3 | BMC Molecular Biology

Figure 3

From: Specificity of DNA-binding by the FAX-1 and NHR-67 nuclear receptors of Caenorhabditis elegans is partially mediated via a subclass-specific P-box residue

Figure 3

Binding of FAX-1 to monomeric and heteromeric strong/weak sites. A. EMSA of FAX-1 protein binding to MON1 sequences (single AAGTCA binding site). Competition with 10-fold molar excess of unlabeled MON1 and DR1A DNA sequences did not reduce non-specific background bands. Similar results (not shown) were obtained with MON2 sequences (single AAGTCA site in different position). B. EMSA of FAX-1 protein binding to HRSW sequences (AAGTCA strong binding site followed by AATTCA weak binding site). Binding could be competed with 10-fold and 100-fold molar excess of unlabeled competitor HRSW DNA, but not DRNC DNA (wedges). Although we obtained strong shifted bands, the proportion of labeled DNA shifted was considerably less than that observed with DR1A sequences (Table 1). We obtained similar results (not shown) with HRWS DNA (AATTCA weak site followed by AAGTCA strong site).

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BMC Molecular Biology

ISSN: 1471-2199

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