Skip to main content
Figure 2 | BMC Molecular Biology

Figure 2

From: Specificity of DNA-binding by the FAX-1 and NHR-67 nuclear receptors of Caenorhabditis elegans is partially mediated via a subclass-specific P-box residue

Figure 2

Binding of FAX-1 to DR1 dimeric sites. A. EMSA of FAX-1 protein binding to DR1G sequences (AGGTCA direct repeats), showing supershift of bands in 5% and 20% mouse anti-FAX-1 antiserum [31]. B. EMSA of FAX-1 protein binding to DR1A sequences (AAGTCA DR1) and failing to bind to DRNC sequences (AATTCA repeats). Binding could be competed with 10-fold and 100-fold molar excess of unlabeled competitor DR1G and DR1A DNA, but not DRNC DNA (wedges). Similar results (not shown) were obtained using radioactively-labeled DR1G DNA (AGGTCA DR1). C. and D. EMSA of FAX-1 protein binding to DR1T sequences (ATGTCA DR1) and DR1C sequences (ACGTCA DR1), respectively.

Back to article page